Fibroblast Response to Nanocolumnar TiO 2 Structures Grown by Oblique Angle Sputter Deposition

Cells are established to sense and respond the properties, including nano- microscale morphology, of substrate they adhere to, which opens up possibility tailor bioactivity. With this background, potential tilted TiO2 nanostructures grown by oblique angle sputtering affect fibroblasts with particular focus on inducing anisotropy in cell behavior is explored. By depositing at different angles relative normal, morphologies, columnar tilt angle, roughness, distances between neighbored nanocolumns can be adjusted. To assess bioactivity resulting structures, L929-mouse seeded vitro nanostructured substrates. Angle-dependent movement velocity distributions cells differently columns a smooth reference sample studied. Cell proliferation rates areas additional factors provide information about viability well-being cells. It could shown that local topography surface has an influence directed